Analysis of the role of vaccinia virus H7 in virion membrane biogenesis with an H7-deletion mutant.
نویسندگان
چکیده
Essential vaccinia virus genes are often studied with conditional-lethal inducible mutants. Here, we constructed a deletion mutant lacking the essential H7R gene (the ΔH7 mutant) with an H7-expressing cell line. Compared to an inducible H7 mutant, the ΔH7 mutant showed a defect at an earlier step of virion membrane biogenesis, before the development of short crescent-shaped precursors of the viral envelope. Our studies refine the role of H7 in virion membrane biogenesis and highlight the values of analyzing deletion mutants.
منابع مشابه
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عنوان ژورنال:
- Journal of virology
دوره 87 14 شماره
صفحات -
تاریخ انتشار 2013